Coding

Part:BBa_K3788009

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)


Colicine A: c.1_1113delins GFP

Colicine are toxins produce by E. coli, among her the Colicine A (ColA). In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1348
    Illegal XhoI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1424
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694
    Illegal SapI.rc site found at 1178


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Categories
Parameters
None